Thermostable beta-glucosidase b

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August undefined, 2024

WebbDescription. Beta-glucosidase deficiency is an autosomal recessive disease resulting in a gangliosidosis called “Gaucher disease,” which is quickly fatal in infants but progresses more slowly in older children. Beta-glucosidase is an enzyme found in peripheral blood leukocytes that normally metabolizes the glycolipid glucocerebroside. Webb6 apr. 2015 · Cel3a was thermostable with an optimum temperature of 71.5 degrees C and half life of 62 min at 65 degrees C and was a specific beta-glucosidase with no beta …

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http://home.konkuk.ac.kr/~deokkun/publication/pub_01.html Webb17 feb. 2012 · This work found a thermostable native β-glucosidase (nBgl3), which is secreted by the lignocellulose-decomposing fungus Aspergillus fumigatus Z5, which was purified to electrophoretic homogeneity and cloned based on the internal sequences obtained from the LC-MS/MS results. top cyber stocks

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WebbThis manuscript provides the first report of the production of ginsenoside 20(S)-Rg3 by a highly thermostable beta-glucosidase. (C) 2015 Elsevier B.V. ... Overexpression and characterization of a Ca2+ activated thermostable beta-glucosidase with high ginsenoside Rb1 to ginsenoside 20(S) ... WebbThe optimal temperature and pH of the plant-expressed BglB was 80 degrees C and 4.5, respectively. BglB activity was preserved in leaves after lyophilization, but decreased by … WebbGenetic potential and expression of beta-glucosidase genes were studied in the topsoil of a Picea abies forest in two contrasting seasons. These seasons were the summer, representing the peak of plant photosynthetic activity, and late winter, after an extended period with no photosynthate input. top cyberwarfare countries

Enzymatic transformation of ginsenoside Rb1 to ginsenoside …

WebbBaohuoside I, a minor flavonoid component of Herba Epimedii, has better bioactivities than its precursor compound icariin. In this work, we have fused the linker (4LP) to thermostable β-glucosidase (Tpebgl3) and successfully prepared the immobilized enzyme (4LP-Tpebgl3@Na-Y) to produce baohuoside I from icariin. Webb12 apr. 2024 · The rare ginsenoside Compound K (CK) is an attractive ingredient in traditional medicines, cosmetics, and the food industry because of its various biological activities. However, it does not exist in nature. The commonly used method for the production of CK is enzymatic conversion. In order to further improve the catalytic … top cyber threats for 2022WebbA thermostable β-glucosidase of particular biotechnological applicability is found in Paecilomyces thermophila. This enzyme, in addition to β-glucosidase activity, displays … top cyber threats to higher education

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Thermostable beta-glucosidase b

Engineering a thermostable Halothermothrix orenii β-glucosidase …

Webb17 apr. 2007 · Thermostable beta-glucosidase B Gene bglB Status UniProtKB reviewed (Swiss-Prot) Organism Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum) Amino acids 755 Protein existence Evidence at protein level Annotation … WebbFactors that give enzymes stability, activity, and substrate recognition result from the combination of few weak molecular interactions, which can be difficult to study through rational protein engineering approaches. We used irrational random mutagenesis and in vivo selection to test if a β-glycosidase from the thermoacidophile Saccharolobus …

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Webb9 juni 2024 · Beta-glucosidase is an enzyme that catalyzes the hydrolysis of the glycosidic bonds of cellobiose, resulting in the production of glucose, which is an important step for the effective utilization of cellulose. In the present study, a thermostable β-glucosidase was isolated and purified from the Thermoprotei Thermofilum sp ... Macromolecules WebbEffects of galactose and glucose on hydrolysis reaction of a thermostable beta-galactosidase from Caldicellulosiruptor saccharolyticus. Appl. Microbiol. Biotechnol. 85(5), 1427-1435 (2010) 120. A. R. Park, D. K. Oh. Galactooligosaccharide production using microbial β-galactosidase: current state and perspectives.

Webb10 dec. 2012 · Biochemical characterization and mechanism of action of a thermostable beta-glucosidase purified from Thermoascus aurantiacus. Biochemical Journal, 353 … Webb1 apr. 1993 · The native beta-glucosidase has a molecular mass of 230 +/- 20 kDa, composed of 58 +/- 2-kDa subunits. The enzyme has a pI of 4.40. Thiol groups are not …

WebbThe gene encoding a thermostable beta-glucosidase (cel3a) was isolated from the thermophilic fungus Talalaromyces emersonii by degenerate PCR and expressed in the filamentous fungus Trichoderma reesei. The cel3a gene encodes an 857 amino acid long protein with a calculated molecular weight of 90.59 … Webb15 feb. 2012 · Of the reported β-glucosidases, P. furiosusβ-glucosidase exhibited the highest thermostability, k (cat), k (cat)/K (m), yield, and productivity for hydrolyzing genistin. These results suggest...

Webb1 jan. 1995 · Thermostable beta-glucosidases In book: Enzymatic Degradation of Insoluble Carbohydrates (pp.197-207) Publisher: American Chemical Society, Washington, DC Editors: J. N. Saddler, M. H. Penner...

picture frames with museum glassWebb11 apr. 2024 · Cyanobacteria are rich sources of secondary metabolites and have the potential to be excellent industrial enzyme producers. β-glucosidases are extensively employed in processing biomass degradation as they mediate the most crucial step of bioconversion of cellobiose (CBI), hence controlling the efficiency and global rate of … top cycle bikesWebbThis manuscript provides the first report of the production of ginsenoside 20(S)-Rg3 by a highly thermostable beta-glucosidase. (C) 2015 Elsevier B.V. ... Overexpression and … picture frames with musicWebb16 dec. 2011 · In this paper, we report a novel thermostable variant of a family 3 β-glucosidase to be an efficient catalyst in alkyl-glucoside forming reactions using transglycosylation with hexanol or octanol ... picture frames with names carvedWebb28 sep. 2001 · A β-glucosidase gene (bglB) of an extremely thermophilic eubacterium, Thermotoga maritima was expressed in Esherichia coli to yield the active enzyme. The … picture frames with oval mattingWebbThe selectivity of alcoholysis compared to hydrolysis was higher than for other reported enzymes. Both beta-glucosidases were also tested in reactions cleaving off glucose … picture frames with no backWebb1 juni 2024 · Beta-glucosidase (BGL; EC 3.2.1.21) catalyzes the hydrolysis of β (1–4) glycosidic linkages. In addition to hydrolytic activity, BGL also exhibits … top cycle company in india