Webb5 maj 2024 · Primary neonatal (3 days old) extrahepatic cholangiocytes or a small intrahepatic cholangiocyte cell line (SBEC) were used for 2D or 3D spheroid culture as … Webb27 nov. 2003 · Aims: We have shown that large and small cholangiocytes, which reside primarily in large and small intrahepatic bile ducts, respectively, have different functions and responses to injuries. However, there are no systematic studies of the molecular differences between small and large cholangiocytes, which would explain cholangiocyte …
After damage of large bile ducts by gamma-aminobutyric acid, …
Webb25 aug. 2024 · Small cell cholangiocytes (SMCCs) were plated at 2 × 10 5 cells/well in a six-well plate and transfected with 2.5 µg control or Wnt7b plasmid, described elsewhere, and 50 µM negative or β-catenin small interfering RNA (siRNA; Thermo Fisher Scientific, Waltham, MA) using Lipofectamine 3000 (Invitrogen, Carlsbad, CA), as per the … WebbSmall and large cholangiocytes differ in nuclear-to-cytoplasmic ratio; small cholangiocytes have a higher ratio, suggesting they are less differentiated and have more capacity for … how many lines of code in github
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WebbSmall and large cholangiocytes differ in nuclear-to-cytoplasmic ratio; small cholangiocytes have a higher ratio, suggesting they are less differentiated and have more capacity for plasticity as compared to large cholangiocytes ( Benedetti et al., 1996; Marzioni et al., 2002 ). WebbThis cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. The cells grow in aggregates, thus cell counts are not accurate. The cells stain positively for cytokeratins. The line can be adapted to grow in shaker flask or spinner flask systems. The N-myc … WebbCa(2+)-dependent protein kinase C (PKC) regulates small cholangiocyte function. Because gamma-aminobutyric acid (GABA) affects cell functions by activation of both Ca(2+) signaling and inhibition of AC, we sought to develop an in vivo model characterized by large cholangiocyte damage and proliferation of small ducts. how are brake discs made