Phm6 vector

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WebbThe HA-AS160(1–532) construct was obtained by PCR amplification with primers containing KpnI and EcoRI restriction sites and cloned into the pHM6 vector (Roche Applied Science). Webb16 juni 2014 · OK cells were transiently transfected with pHM6-DNPEP, pHM6-(H94F) DNPEP, or pHM6 empty vector (control) using Lipofectamine 2000 (Invitrogen). Forty-eight hours posttransfection, conﬂuent OK cell monolayers were washed three times in ice-cold PBS, and then biotinylated with EZ-Link NHS-SS- Biotin (Pierce) at 4°C with gentle agitation.

Chloride channel ClC-5 binds to aspartyl aminopeptidase to …

WebbPhm6 Expression Vector, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … WebbThe S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click … chromosomes dna function biology

Addgene: Vector Database - pHM6

WebbExpression Plasmid Vector Phm6, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … WebbMammalian Expression Vectors— pHM6 was used to express molecules tagged at the N terminus with hemagglutinin (HA) in mammalian cells (driven by a cytomegalovirus immediate early promoter). Construction of pHM6-Cezanne 1–858 and pHM6-Cezanne 1–443 has been described previously ( 11 ). WebbpHM6 87228 ™ Organism Yersinia enterocolitica subsp. enterocolitica (Schleifstein and Coleman) Frederiksen Clone type Clone Applications Molecular biology Product format … chromosomes duplicate during metaphase

Cloning of M and NP gene of H5N1 avian influenza virus and …

Enzymatic activity of tobacco transplastomic plants. (a, d) …

WebbWe identified p90 ribosomal protein S6 kinases (RSKs; also known as MAPK-activated protein kinases, MAPKAP-K1/K2) as the kinase necessary for Thr 198 phosphorylation. RSK-dependent phosphorylation induced 14-3-3 binding and cytoplasmic localization of p27 Kip1 through a Thr 198 phosphorylation-dependent mechanism. Webb17 juni 2005 · Human wild-type ILK cDNA (WT-ILK) in a pFLAG-CMV-2 vector and a pHM6 vector were established in our laboratory. Several ILK fragments that encompassed residues 1–406 (ΔC407- ILK ) and 1–375 (ΔC376- ILK ) were generated by converting appropriate codons in WT- ILK cDNA to stop codons using a QuickChange site-directed … chromosome segment substitution linesWebbPlasmid pHM6-alphasynuclein-WT from Dr. David Rubinsztein's lab contains the insert SNCA and is published in Biochem J. 2000 Mar 15;346 Pt 3:577-81. This plasmid is … chromosomes easy drawing

"WebbpET SUMO (linearized) Linearized bacterial vector with 3'-T overhangs for TA cloning and inducible expression of an N-terminally 6xHis- and SUMO-tagged protein. Sequence … " - Phm6 vector

Phm6 vector

Webb1 apr. 2015 · Opossum kidney (OK) cells were transfected with pHM6 empty vector (control) or a pHM6-DNPEP construct (DNPEP) and harvested 48 h later. Measurements of monomeric (G) and total cellular actin was performed by DNase I inhibition assay to determine the G/F-actin ratios, the ratio of F-actin content to total cellular protein, and … Webb16 sep. 2009 · The expression plasmid containing the M gene (pHM6-m) or the NP gene (pHM6-np) was then constructed by inserting the M or NP gene into the pHM6 eukaryote …

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WebbPlasmid pHM6-alphasynuclein-A53T from Dr. David Rubinsztein's lab contains the insert SNCA and is published in Biochem J. 2000 Mar 15;346 Pt 3:577-81. This plasmid is … Webb16 feb. 2016 · d pHM6 containing celB gene for cloning in rrn16 and 3′rps12 homologous recombination site into chloroplast genome; these vectors contain aadA as selection …

WebbInvolvement of 3-Phosphoinositide-dependent Protein Kinase-1 in the MEK/MAPK Signal Transduction Pathway* Received for publication, February 25, 2004, and in revised form, June 1, 2004

WebbpHM6 (Search Vector Database) Backbone manufacturer Roche Backbone size w/o insert (bp) 5450 Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance … WebbBasic Vector Information; Vector Name: pHM6: Antibiotic Resistance: Ampicillin Length: 5450 bp: Type: Mammalian Expression Vectors Selection Marker: …

Webb15 juni 2003 · Oligonucleotides carrying Bsu15I and Psp5II restriction sites were introduced in SacII-NotI sites of pHM6 vector. The cDNA encoding the N-terminal part of PTPL1 was cloned by PCR using pSV7d-PTPL1 (gift from C. H. Heldin, Ludwig Institute for Cancer Research, Uppsala, Sweden) as a template in Sac II- Bsu 15I sites of modified pHM6 …

WebbThe product is provided 'AS IS' and the viability of ATCC ® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the … chromosome selectionhttp://www.protocol-online.org/biology-forums/posts/5295.html chromosome sexuel hommeWebbVector Database. Digital collection of empty plasmid backbones from publications and commercially available sources. Help Center . Ordering & MTAs. How do I place an … chromosomes easy definitionWebb1 aug. 2008 · The HA-tagged protein was obtained from a pHM6 vector encoding no protein (Mock). HA-tagged Identification of the domains responsible for interactions between Freud-1/Aki1 and PDK1. (A) The... chromosomes first become visible inWebbcloned into blunted HindIII(5=)-BamHI(3=) sites of expression vector pHM6 (Invitrogen, Carlsbad, CA), while the 988 bp rat mas PCR fragment was cloned into HindIII (5=)-EcoRI (3=) sites of pHM6 after digestion with HindIII and EcoRI. Chemicals. Mas agonist (AR234960) and inverse agonists (AR244555 and AR305352) were synthesized at Arena … chromosomes find each other and synapseWebba pc5FLAG vector was generated by polymerase chain reaction (PCR) with an I.M.A.G.E clone (clone ID 6169151; Open Biosystems, Huntsville, AL, USA) as the template. The p60 c-src cDNA in a pHM6 vector was generated by PCR with an I.M.A.G.E clone (clone ID 4548399; Open Biosystems) as the template. Deletion mutants of TUSC4 were … chromosomes femaleWebb15 apr. 2010 · The PCR amplified merB::flag fragment (0.8 kb) was subcloned into the binary expression vector pHM6, downstream of the E12 promoter, the Cauliflower mosaic virus 35 S promoter with two enhancers in tandem, and an omega sequence, to create the plasmid pBMK01. chromosomes first become visible during