WebbpH-nCas9-PPE-V2. Plasmid #170131. Purpose. For plant prime editing in rice plants or monocotyledons protoplasts. Depositor. Caixia Gao. ... Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. WebbPlasmid pH-nCas9-PPE from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 May;38(5):582-585. doi: 10.1038/s41587-020 …
What is dCas9? And what is its application? AAT Bioquest
Webb24 mars 2024 · PPE-NC-v1 showed the highest editing efficiencies, ranging from 0.2% to 19.5% in both rice and wheat protoplasts, followed by PPE-NC-v2 and PPE-∆RNase H (Fig. 1f,g ). These three new PPEs... Webb14 mars 2024 · After incubation at room temperature for 12 h, protoplasts were harvested and homogenized in 100 μl lysis buffer containing 25 mM Tris–HCl, pH 7.8, 2 mM DTT, 2 mM trans -1,2-diaminocyclohexane- N ’ N ’ N ’ N ’-tetraacetic acid, 10% (v/v) glycerol, 1% (v/v) Triton X-100. song keeping up with the joneses faron young
PlantPegDesigner/README.md at master · …
WebbThe pOsU3, pTaU3, and pTaU6 vectors are recommended for particle bombardment and protoplast transformation experiments. The pH-nCas9-PPE-V2 vector is recommended … WebbpH-nCas9-PPE-V2 Sequences (1) Addgene Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated … Webb24 mars 2024 · pH-ePPE: a plant nCas9-RT and pegRNA expression vector that includes an OsU3 promoter-driven SpCas9-based sgRNA scaffold and a ZmUbi promoter-driven … smallest country in square miles