WebA list of cross-contaminated or misidentified cell lines. 1 reference. Isolation and propagation of the animal rotaviruses in MA-104 cells--30 years of practical experience. 1 reference. … WebIsolation of Porcine reproductive and respiratory syndrome virus (PRRSV) on MA104 or MARC-145 cells is frequently used in PRRS diagnosis. However, the ability of recent field isolates to grow on these established simian cell lines has not been determined.

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WebSep 25, 2012 · MA104 cells on coverslips were transfected with plasmids expressing WT NSP4-EGFP or NSP4-ASDASA-EGFP as previously described . MA104 cells were either loaded with 50 μM BAPTA-AM at 4 h posttransfection or maintained in normal medium. At ∼20 h posttransfection, a subset of the transfected cells was treated with 1 μM TG for 3 … WebJul 25, 2024 · Parental MA104 cells were transduced with the shRNA-expressing constructs and at 72 hrs post-transduction the cells were passaged in the presence of 30 µg/mL blasticidin to select for stably ... fender acoustic guitar with amp

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WebCytotoxicity of 18β-GA on MA104 cells. MA104 cells were digested and dispersed, inoculated into 96 well plates at 1 × 10 6 cells/mL. Further, they were cultured at 37 °C for 24 h with 5% CO 2. Different concentrations of 18β-GA (1, 2, 4, 8, 16, 32, 64, and 128 μg/mL) were added to each well, and a normal cell control was established. WebFeb 11, 2024 · The results indicate that, at least in cell culture, human VP7 has a high reassortment potential, while reassortment of human VP4 from unadapted human RVA strains with simian RVA seems to be limited. ... detail by sequencing the whole genome of the reassortants before and after a large number of sequential passages in MA104 cells. … WebThe MA104 cells were co-transfected with the indicated plasmids. The transfected cells were lysed and clarified by centrifugation at 160 ×g at 4 °C for 15 min. The supernatants were incubated with anti-Flag or anti-HA antibodies and rotated at 4 °C overnight. dehidrated instant icecream