Chromotek gfp-trap

WebThe ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. £198.00 - £4914.00 Specifications View More Specs Products 5 Description Specifications Description Comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads Specifications WebThe ChromoTek GFP-Trap® is a ready-to-use affinity resin for immunoprecipitation (IP) of GFP-fusion proteins. GFP-Trap consists of an anti-GFP Nanobody/ V H H coupled to agarose beads, magnetic agarose beads, magnetic particles or multiwell plates. Specificity. GFP, EGFP, CFP, YFP, BFP and many more GFP derivatives, see: Fluorescent protein ...

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WebApr 4, 2024 · For pull down of GFP, GFP Trap (ChromoTek) beads were washed with PBS and lysis buffer before incubation with whole cell extracts. For pull down of NR2F2 Isoform 1, Dynabeads Protein A (Invitrogen ... WebDec 24, 2015 · The precipitate was washed six times with extraction buffer and eluted in 2× Laemmli buffer. Anti-GFP Trap-A beads (gta-10; Chromotek) were used for IP. For protein immunoblots, proteins were separated by 12% SDS-PAGE and transferred to polyvinylidene fluoride membrane (Roth) by semidry blotting in 25 mM Tris-HCl, pH 8.3, 150 mM … opening sheet delhi high court https://itshexstudios.com

Proteintech ChromoTek GFP-Trap™ Magnetic Agarose

WebMay 25, 2024 · ChromoTek GFP-Trap ® is optimized for the immunoprecipitation of GFP-tagged proteins and their interacting factors. The GFP-Trap consists of the ChromoTek anti-GFP Nanobody/ V H H … WebApr 10, 2024 · Total cell extracts of yeast cells untreated or treated with CHX were incubated in ChromoTek GFP-Trap Agarose beads (Proteintech) for 2.5 h at 4 °C to pull-down AGP2-GFP tagged-associated proteins. After incubation, the beads coupled to proteins were washed with washing buffer (10 mM Tris/Cl pH 7.5, 0.5 mM EDTA, 150 … WebGFP-Trap® Magnetic Agarose Kit Product code: gtmak-20 Contact [email protected] ChromoTek GmbH Am Klopferspitz 19 82152 Planegg-Martinsried Germany phone: +49 89 124 148 80 fax: +49 89 124 148 811 ChromoTek Inc. 62-64 Enter Lane Islandia, NY 11749 USA phone: 631 501 1058 fax: 631 501 1060 … iowvoe led mirror

Nano-Traps for superior immunoprecipitation

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Chromotek gfp-trap

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Chromotek gfp-trap

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WebAug 27, 2024 · ChromoTek GFP-Trap ® is optimized for the immunoprecipitation of GFP-tagged proteins and their interacting factors. The GFP-Trap consists of the ChromoTek anti-GFP Nanobody/ V H H … WebSep 16, 2024 · ChromoTek GFP-Trap® magnetic agarose affinity beads ( see Note 2 ). 2. Low protein binding microcentrifuge tubes (e.g., LoBind® tubes). 3. Magnetic separation rack (should fit 1.5-ml microcentrifuge tubes). 4. Table-top centrifuge (cooled, with rotors suitable for 1.5- and 2-ml microcentrifuge tubes, RCF:19,000 × g ). 5.

WebAlpaca GFP-Trap® is the top selling GFP antibody as it allows for one-step pulldowns that can be directly used in downstream applicaitons such as enzyme activity assays or mass … WebSep 7, 2024 · ChromoTek GmbH (Munich, Germany) offers nanobody based real time assays to visualize and measure the action of candidate drugs or other substances of interest on important regulatory processes in living cells. Presently, four Chromobody ® plasmids are available for analyzing the cell cycle, apoptosis, DNA methylation and …

WebFeb 12, 2024 · GFP-Trap is stable in harsh and stringent conditions. This allows you to set the binding condition so that only the protein of interest is bound. ChromoTek lists the following conditions that can be applied to … WebThe ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to …

WebChromoTek GmbH. F2H®-Kit Basic: Kit comprises F2H®-BHK Cell Line, 1 vial 5 X 106 frozen cells (f2h-bhk); 100 µg Platform Reagent (f2h-p100); and 30 µg Control-DNA (f2h-c30) Platform Reagent: Transfection supplement enabling assembly of the nuclear PPI-platform to anchor GFP-tagged proteins; for 125 transfections (24-well format) or 500 ...

http://alpaca.chromotek.com/ opening sheet for civil revisionWebChromoTek Nano-Traps are the benchmark in immunoprecipitation. They guarantee fast, reliable and effective pull-downs of proteins and interacting partners, and have been cited in 4,000+... iow water coolerWebThe ChromoTek GFP-Trap®Agarose consists of an anti-Green Fluorescent Protein (GFP) Nanobody (VHH), which is covalently bound to agarose beads. GFP-Trap Agarose is … i.o.w weather 10 day forecastWebApr 10, 2024 · Cell lysates were subjected to GFP-trap. The WCLs and GFP-trap products were immunoblotted for the indicated proteins and phospho-proteins. c, d, HEK293T cells expressing Ctrl or ILF3 shRNAs... iow water supplyWebJun 23, 2024 · Split GFP technologies to structurally characterize and quantify functional biomolecular interactions of FTD-related proteins. Sci Rep. 2024;7:14013 pubmed publisher Shwab E, Juvvadi P, Waitt G, Soderblom E, Moseley M, Nicely N, et al . iow water companyWebManufacturer Chromotek GmbH 5.0 / 5.0 2 reviews Write your own review GFP -Trap® - use your fluorescence fusion as tag for … iow walking routesWebHello, you can melt Chromotek GFP-TRAP at 95*С and use magnet to remove magnetic agarose beads as you described without problem. Cite 3 Recommendations 21st Jan, 2024 Adam Thiesen Massachusetts... iowwa real estate investment club